1:00 – 1:10 p.m.
WELCOME AND OVERVIEW
Tereza Martinu, MD, MHS, University of Toronto, Toronto, Canada
1:10 – 2:10 p.m.
SESSION 1 – ADAPTIVE IMMUNITY IN THORACIC TRANSPLANTATION
Chair: Esme Dijke, PhD, University of Alberta, Edmonton, Canada
1:10 p.m. – Overview of Adaptive Immunity in Transplantation: Attack of the Clones (15 minutes)
Federica Meloni, MD PhD, Policlinico San Matteo, Pavia, Italy
Teaching/Discussion Points
- T/B cell receptors, diversity of specificities
- Antigen presentation: Intracellular and extracellular antigens and direct versus indirect alloimmunity
- Compare pathology and epidemiology of acute cellular and antibody-mediated rejection in heart and lung transplant
1:25 p.m. – T cells: They Pity the Fool that Don't be Cool (15 minutes)
Carla Baan, PhD, Erasmus Medical Center, Rotterdam, The Netherlands
Teaching/Discussion Points
- Thymic development and T cell signaling
- Why are there alloreactive T cells in the first place?
- Major T cell types and their roles: CD8, CD4, Tregs, central/effector memory, tissue resident vs. lymphoid
- Cytokines and their associated helper T cell subsets and their roles: Th1, Th2, Th17
- T cell Metabolome
1:40 p.m. – B cells: You too Can be a B-liever (15 minutes)
Simon Urschel, MD PhD, University of Alberta, Edmonton, Alberta, Canada
Teaching/Discussion Points
- How does antibody-mediated rejection happen?
- From co-stimulation to complement and antibody-dependent cellular cytotoxicity, with attention to therapeutic targets
- B cells in antigen presentation
- Immune protection from B cells (suppression, cytoprotection, immune regulation)
1:55 p.m. – Panel Discussion – What cell is most important in acute rejection? (15 minutes)
2:10 – 2:15 p.m.
BREAK
2:15 – 3:45 p.m.
SESSION 2 – INNATE IMMUNITY IN THORACIC TRANSPLANTATION
Chair: John Greenland, MD PhD, UCSF, San Francisco, CA, USA
2:15 p.m. – Overview of Innate Immunity in Transplantation: Every Body Needs a Hero (15 minutes)
Glen Westall, MD PhD, Alfred Hospital, Melbourne, Australia
Teaching/Discussion Points
- The various barriers of innate immunity (physical, chemical and cellular)
- Signaling pathways (TLR, PRR, DAMPs, PAMPs)
- Interaction of complement and innate immune cells with adaptive immunity
- The role of innate immunity in ischemia-reperfusion injury
- Contrast lung versus heart-specific concepts.
2:30 p.m. – Neutrophils: The First Responders (15 minutes)
Andrew Gelman, PhD, Washington University, St-Louis, MO, USA
Teaching/Discussion Points
- Characteristics of neutrophils and role in inflammation
- Neutrophil extracellular traps (NETs)
- The role of neutrophils in transplantation
2:45 p.m. – Macrophages and Dendritic Cells: The Usual Suspects (15 minutes)
Stephen Juvet, MD PhD, University of Toronto, Toronto, Ontario, Canada
Teaching/Discussion Points
- Characteristics of macrophages and DCs
- Phagocytosis, antigen processing and presentation
- The role of DCs and macrophages in inflammation and transplantation
3:00 p.m. – Innate Lymphoid Cells: The Unusual Suspects (15 minutes)
Christine Falk, PhD, Hannover Medical School, Germany
Teaching/Discussion Points
- Characteristics of ILCs, NK cells and T cells
- The role of ILCs, NK cells and T cells in inflammation and transplantation
- Immune memory in innate immunity
3:15 p.m. – Panel Discussion – What cell is most important to PGD (15 minutes)
3:30 – 4:30 p.m.
SESSION 3 – FIBROSIS AND TRANSPLANT TOOLS OF THE TRADE
Chair: Tereza Martinu, MD, MHS, University of Toronto, Toronto, Canada
3:30 – Transplantation Endgame: Fibrosis Pathways (15 minutes)
Stijn Verleden, PhD, University of Antwerp, Antwerp, Belgium
Teaching/Discussion Points
- The roles and manifestations of fibrosis in chronic rejection of the heart and lung
- Triggers of fibrosis
- Injury and dysregulated repair
- Pro-fibrotic factors; Fibroblast phenotypes
- Extracellular matrix; Origins of fibroblasts (local proliferation and migration, fibrocytes, epithelial-mesenchymal transition)
3:45 p.m. – Cellular Assays: The Sixth Sense (15 minutes)
Carla Baan, PhD, Erasmus Medical Center, Rotterdam, The Netherlands
Teaching/Discussion Points
- Describe the concepts behind high parameter flow cytometry and mass cytometry, Seahorse assay, confocal and intravital microscopy
- Focus on the use of these tools for the study of acute cellular rejection
4:00 p.m. – Mouse Modeling in Thoracic Transplantation: Secrets of NIMH (15 minutes)
Stephen Juvet, MD PhD, University of Toronto, Toronto, Ontario, Canada
Teaching/Discussion Points
- Describe approaches to designing mouse model-based studies
- What controls need to be considered
- Genetically-engineered mice
- Focus on the use of these tools for the study of chronic rejection.
4:15 p.m. – Q&A Discussion (10 minutes)
4:25 – 4:30 p.m.
CLOSING REMARKS
Tereza Martinu, MD MHS, University of Toronto, Toronto, Ontario, Canada